EliKine™ Rat IL-4 ELISA Kit from Abbkine

November 02 01:45 2018
EliKine™ IL-4 Rat ELISA Kit, Calibration range: 1.56 pg/mL-100 pg/mL, Sensitivity: 1.0 pg/mL. $220/96T, in stock.

Application notes:

EliKine™ Rat IL-4 ELISA Kit employs a two-site sandwich ELISA to quantitate IL-4 in samples. An antibody specific for IL-4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-4 is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

IL-4 rat ELISA Kit components:

1. Rat IL-4 microplate: 96 well polystyrene microplates (12 strips of 8wells) coated with the antibody specific for Rat IL-4.

2. Rat IL-4 standard: Rat IL-4 in a buffered protein base with preservatives, lyophilized.

3. Standard diluent: Diluent solution for dilute standards and samples.

4. Assay Buffer: Diluent solution for dilute biotin-conjugated antibody and Streptavidin-HRP.

5. Biotin-Conjugate Rat IL-4 detection antibody: Biotin-conjugated Rat IL-4 detection antibody. 100× liquid.

6. EliKine™ Streptavidin-HRP: 100× liquid.

7. HRP substrate: TMB (Tetramethyl-benzidine) solution.

8. Stop solution: 2N sulfuric acid.

9. Wash buffer: PBS with 1%Tween-20; 20× liquid.

10. Plate covers.

Assay protocol summary:

Typical data:

Detection range: 1.56 pg/mL-100 pg/mL

Sensitivity: The minimum detectable dose (MDD) of Rat IL-4 is typically less than 1.0 pg/mL

Abbkine offers $140/48T, $220/96T, $1000/96T*5 three sizes for your choice on online shopping at KET9003

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